Measuring volume perturbation of proximal tubular cells in primary culture with three different techniques
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چکیده
منابع مشابه
Measuring volume perturbation of proximal tubular cells in primary culture with three different techniques.
Osmotic cell volume perturbations of rabbit proximal tubule (PT) in primary culture were measured using three independent techniques. Automatic cell thickness monitoring of PT monolayers revealed that cell volume rapidly increased by 39 +/- 2% in hypotonic medium (150 mosM), which was followed by partial regulatory volume decrease (RVD). Subsequent incubation in hypertonic medium (500 mosM) rap...
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Since in vitro cell culture models often show altered apical transporter expression, they are not necessarily suitable for the analysis of renal transport processes. Therefore, we aimed here to investigate the usefulness of primary-cultured rat proximal tubular cells (PTCs) for this purpose. After isolation of renal cortical cells from rat kidneys, PTCs were enriched and the gene expression and...
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A simple method is described to establish primary cultures of kidney proximal tubule cells (PTC) on membranes. The permeable membranes represent a unique culture surface, allowing a high degree of differentiation since both apical and basolateral membranes are accessible for medium. Proximal tubule (PT) segments from collagenase-digested mouse renal cortices were grown for 7 days, by which time...
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چکیده ندارد.
15 صفحه اولProtein kinase C activation has dissimilar effects on sodium-coupled uptakes in renal proximal tubular cells in primary culture.
Protein kinase C (Ca2+/phospholipid-dependent enzyme) was shown to be present in renal brush border membranes. To evaluate the influence of protein kinase C activation on three apical transport systems, we studied the effect of phorbol myristate acetate (PMA) and of two diacylglycerol analogs, oleoylacetylglycerol and dioctanoylglycerol, on sodium-dependent uptakes of phosphate (Pi), L-alanine,...
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ژورنال
عنوان ژورنال: American Journal of Physiology-Cell Physiology
سال: 1996
ISSN: 0363-6143,1522-1563
DOI: 10.1152/ajpcell.1996.271.1.c235